Quantitative Real Time Polymerase Chain Reaction (RT-qPCR)

Quantitative Real Time Polymerase Chain Reaction (RT-qPCR) QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTION (RT-qPCR) Groundworks Every single preliminary succession were planned utilizing the online instrument Primer 3-BLAST (NCBI) and the groundworks were acquired from Sigma Aldrich, Bangalore, India. Relative articulation of changing development factor beta (TGF-ÃŽ ²), myosin overwhelming chain beta (ÃŽ ²-MHC), endothelial nitric oxide synthase (eNOS) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) was considered. Forward and invert introductions for the above qualities were utilized for intensification. Table 5. PCR Primer subtleties RNA segregation All dish sets were flushed with diethyl-pyrocarbonate (DEPC) offered water restrain RNases. All out RNA was separated utilizing guanidium thiocynate-chloroform-phenol technique for Chomczynski and Sacchi (1987). Absolute RNA disconnection unit (BioUltra, Sigma Aldrich,USA) was used for this investigation Subsequent to cleaning with saline, heart and aorta tissues were homogenized in denaturing arrangement with newly included ÃŽ ²-mercaptoethanol. After homogenization 2M sodium acetic acid derivation arrangement (pH. 4.0), water soaked phenol and chloroform: isoamyl liquor (49:1) was included. The blend was shaked energetically and permitted to cool on ice for 15 minutes. The blend was centrifuged at 10,000 Ãâ€"g for 20 minutes at 4 oC. The fluid stage was moved in a new cylinder and an equivalent volume of super cold isopropanol was included. RNA was accelerated by putting the example at - 20 oC for 60 minutes. At that point the blend was centrifuged at 10,000 Ãâ€"g for 20 minutes at 4 oC. The pellet was washed with 70% ethanol and RNA was put away in DEPC water at - 80 oC. RNA quality and amount was surveyed by nano-drop spectrometer. Constant PCR enhancement SYBR Green Quantitative RT-qPCR Kit was utilized in this examination and the PCR analyze was completed in eppendorff realplex mastercycler. 1â µg RNA was opposite deciphered by utilizing Molone murine leukemia infection (M-MuLV) invert transcriptase according to fabricates directions. At that point the intensification program (94 oC †45 seconds, toughening †45 seconds, augmentation 72 oC-1 moment) was applied with explicit strengthening temperature. The strengthening temperatures of TGF-ÃŽ ², ÃŽ ²-MHC, eNOS and GAPDH were 58, 52, 55, and 55 oC, separately. The explicitness of the groundworks was affirmed by settling the PCR items in 1.5% agarose gel electrophoresis. The relative overlay change of articulation was determined by standardized the articulation with GAPDH. The RT-qPCR results were measured utilizing the ‘threshold line’ and the ‘cycle threshold’. The ‘threshold line’ is where the response arrives at a fluorescent force above foundation. The cycles at which the examples arrive at this level is known as the ‘cycle threshold’ (Ct). The factual examination of the RT-qPCR results was determined by utilizing the à ¢Ã‹â€ Ã¢â‚¬ Ct = (Ct estimation of quality of intrigue †Ct estimation of GAPDH). Relative quality articulation was acquired by à ¢Ã‹â€ Ã¢â‚¬ à ¢Ã‹â€ Ã¢â‚¬ Ct techniques (à ¢Ã‹â€ Ã¢â‚¬ Ct test †à ¢Ã‹â€ Ã¢â‚¬ Ct of control), with the utilization of the benchmark group as a calibrator for correlation of all obscure example quality articulation levels. The relative quality articulation overlay change was gotten from 2â€Ã ¢Ã‹â€ Ã¢â‚¬ à ¢Ã‹â€ Ã¢â‚¬ Ct (Schmittgen and Livak, 2008). IMMUNOHISTOCHEMICAL LOCALIZATION (IHC) Immunohistochemistry (IHC) IHC was proceeded as depicted by Rocha et al., (2009) utilizing Super Sensitive Polymer-HRP Detection System pack, from Biogenex, USA. The Super Sensitive Polymer-HRP Detection System is an atypical recognition framework utilizing a non-biotin polymeric innovation that utilizes two significant parts: a Poly-HRP reagent and super Enhancerâ„ ¢. As the framework did not depend on the biotin-avidin framework, the issues related with endogenous biotin are totally wiped out. The identification of antigens in tissues by immunostaining is a two-advance procedure. The initial step includes the official of an immune response to the antigen of intrigue and the subsequent advance includes the location and perception of bound counter acting agent by one of an assortment of compound chromogenic frameworks. The decision of location framework will drastically affect the affectability, utility and usability of the technique. Technique Paraffin-inserted tissue was sliced to get areas of around 4  µm thickness. The mounted paraffin-inserted cuts are deparaffinized in xylene and rehydrated utilizing an ethanol/H2O inclination. Warmth interceded antigen recovery step was done for 10 min and afterward the slides were permitted to cool to room temperature for another 20 min. This was trailed by peroxidase square treatment (to square endogenous peroxidase catalyst action) for 10-15 min and afterward power square treatment (to square vague authoritative of antibodies to exceptionally charged destinations) for another 15 min. The areas were hatched with the concerned weakened essential neutralizer answer (for 2 h (1:200)) trailed by treatment with the super enhancer answer (for 30 min) and overly touchy Poly-HRP answer (for 30 mins). After shading improvement with DAB and counterstaining with haematoxylin, the segments were seen under the magnifying lens and photos were taken. TRANSMISSION ELECTRON MICROSCOPIC STUDY The ultrastructure of the heart example was inspected by Transmission Electron Microscopy (TEM) as per the strategy for Lang (1987), by the procedure of slim separating. Reagents Glutaraldehyde arrangement: 3% Osmium tetroxide: 2% osmium tetroxide in 10 mM sodium phosphate cushion, pH - 7.4 Ethanol: 75%, 95% and 100% Uranyl acetic acid derivation: 1% Lead citrate: 3% Sodium phosphate cushion: 0.1 M, pH 7.4 Method Following the penance, the heart tissues were dismembered and fixed with an answer of 3% glutaraldehyde for 2 hours at room temperature and washed threefold with phosphate cushion to evacuate glutaraldehyde. Post-obsession was finished by an answer containing 2% osmium tetroxide in 10mM sodium phosphate cradle and left for the time being. At that point, the osmium tetroxide arrangement was evacuated and supplanted with 75% ethanol. This decreases the rest of the osmium tetroxide to osmium dioxide, which shapes a hasten in the liquor. Following 10 minutes, the liquor was supplanted with a couple of ml of 75% ethanol. Following 30 minutes, the liquor was supplanted with 95% ethanol and left for 30 minutes. This arrangement was supplanted with 100% ethanol and washed threefold and afterward dried in CH3)2CO. After drying out, the tissues were equilibrated for 30 minutes in 1:1 blend of epoxy propane and the implanting medium, epon 812 (additionally called epikote gum 812). A blend of the gum and two solidifying specialists, dodecyl succinic anhydride and methyl anhydride were utilized. A diamine impetus by and large N-benzyl-N-diethylamine was included not long before use. The 1:1 blend was poured off and supplanted with full quality sap. This progression was rehashed a few times to guarantee full penetration of the installing medium. The tissue was then moved to a shaft case with a wooden stick and the container was loaded up with new pitch blend. The wooden stick was utilized to prod the example down to the focal point of the base of the container. Next, the square holder was set with the example in sight-seeing oven at 60 °C for 48 hours to polymerize the pitch totally. When the squares are solidified, they are prepared for segmenting. The closures of the example squares were cut ut ilizing glass blades and ultra slim areas were cut utilizing a LKBUM4 ultramicrotome. The segments were picked upon carbon frameworks and post-recolored with consolidated uranyl and lead stain and washed with refined water and dried. In the wake of drying, the lattices were analyzed under a Philips EM201C transmission electron magnifying lens (Philips, Eindhoven, Netherlands). WESTERN BLOT ANALYSIS Western smearing was performed to dissect the articulation example of eNOS in the aorta and reperfused hearts as indicated by strategy for Laemmli (1970). Standard Following the protein estimation, the examples were isolated utilizing SDS-PAGE gel electrophoresis and the isolated atoms are smudged onto a polyvinylidene fluoride (PVDF) film. In the wake of hindering, the essential counter acting agent was added and permitted to tie to the protein followed by washing (which expels vaguely bound immune response); at that point a catalyst marked optional immunizer was included, to identify the essential neutralizer. The area of the optional immunizer was dictated by including a fitting substrate for the catalyst conjugated to the auxiliary counter acting agent. Reagents Acrylamide stock: 30% acrylamide, 0.8% N,N†²-methylene bisacrylamide Isolating gel cushion: 1.5 M Tris, pH 8.8 Test cushion: 0.5 M Tris, pH 6.8 Sodium dodecylsulfate (SDS): 10% Ammonium per sulfate (APS): (10%) N,N,N,N-tetramethylethylenediamine (TEMED) Isolating gel overlaying arrangement: Water-soaked isobutanol Test Buffer: Tris (0.5M, pH 6.8)- 2.5 mL SDS (10%)- 4.0 mL Glycerol (100%)- 2.0 mL ÃŽ ²-Mercaptoethanol-0.8 mL (or 1 M DDT-0.5 mL) Bromophenol Blue (0.1%)- 300  µL Refined water (400  µl) to 10.0 mL Running gel cradle Tris-6.05 g Glycine: 28.80 g 10% SDS: 10.0 mL or (1.0 g) Refined water to 1000 mL Recoloring arrangement Coomassie splendid blue R250-300 g Methanol-80 mL Acidic corrosive 20 mL Refined water-100 mL Destainning arrangement Acidic corrosive 100 mL Methanol-300 mL Refined water: 1000 mL System The aortic tissues were homogenized in a super cold radio immuno precipitation cushion (RIPA) (1% Triton, 0.1% SDS, 0.5% deoxycholate, 1 mM/L EDTA, 20 mM/L Tris (pH 7.4), 150 mM/L NaCl, 10 mM/L NaF, and 0.1 mM/L phenylmethylsulfonyl fluoride (PMSF)). The homogenate was centrifuged at 10,000 Ãâ€"g for 20 min at 4 °C to expel flotsam and jetsam and the supernatant was utilized to decide the protein grouping of the lysates utilizing the BCA protein test pack (Merck, India). Move of proteins to film Tests containing 50 ÃŽ ¼g of all out cell proteins were loade

Leadership Speech :: essays research papers

Numerous individuals accept that authority is just being the principal, greatest or generally ground-breaking. Authority in associations has an alternate and increasingly significant definition. A pioneer is somebody who sets heading in an exertion or errand and impacts or rouses individuals to follow that course. The force point introduction clarifies initiative is the impact that specific people apply on the objective accomplishment of others in an authoritative setting. At the point when some consider initiative the possibility of the military is considered while others utilize the term to allude to official administration. These are scarcely any instances of various kinds of pioneers there are. There are additionally various jobs of administration, for example, senior-level administrators and center chiefs. Roused pioneers who have charm and elevated levels of enthusiastic strength will be straightforward and have trustworthiness in the way in which they lead. A successful pioneer must be regarded by the individuals from their gathering so as to perform adequately. Another part of administration incorporates initiative qualities, for example, being compelling and rousing. There likewise various settings of administration, e.g, driving oneself, driving others, driving gatherings, driving associations. A few qualities that are regularly connected with being a successful pioneer incorporate a proportion of insight, high vitality, self-assurance, predominance, and a requirement for accomplishment. A compelling, magnetic pioneer must show others how its done. That pioneer must have the option to recognize what is happening, the activity or undertaking that is being cultivated, and have the option to lead the gathering into playing out the job needing to be done effectively. There are a wide range of sorts of speculations on initiative. There is Fiedler’s possibility hypothesis, situational speculations of initiative and House’s way objective hypothesis. These hypotheses are utilized to make an individual or supervisor an increasingly powerful pioneer. They can be utilized as a guide for people and assist them with accomplishing whatever objective is set out by their association or gathering. Fiedler’s Theory is expressed in the force point introduction as the relationship between administration direction and gathering adequacy is dependent upon how good the circumstance is for applying impact. On the off chance that there is some disaster which will come to pass for a gathering or set of people in the event that an undertaking isn't finished, at that point that gathering will be increasingly powerful as they are propelled to finish that errand to turn away that specific fiasco. The Situational Theories of initiative clarify how administration style must be custom fitted to the requests of the undertaking and the characteristics of subordinates.

Your Guide to an Impeccable Curriculum Vitae for Grad School

Your Guide to an Impeccable Curriculum Vitae for Grad School Among the common documents required for graduate school admission, a curriculum vitae (CV) will often be a part of your application paperwork. Not all programs require one but if you plan to go into any sort of advanced academic field, youll still need to create a CV and add to it consistently as you gain experience and publishing credentials. Whether its for a scholarship, a graduate teaching position, or a job after you graduate, having a well-written CV will be just as important as your resume.Your CV will be an important document for graduate school admission and post-grad work.A lot of people get confused between the difference between a CV and a resume. The easiest way to look at it is to understand that the purpose of a CV is to highlight your academic accomplishments and experience, while a resume focuses more on your professional qualifications and work history. There are some elements theyll both haveâ€"such as your contact information, any professional degrees you hold and where you earned themâ€"but for the most part, theyll look like two very different documents.The sections of a CVThe sections youll want to include on a CV for grad school will likely be different than what youd include on a CV for a university teaching position. For example, a CV for a teaching position is commonly divided into the following sections:Personal detailsEducationTeaching ExperienceResearch ExperienceHonors and AwardsGrants AwardedTechnical CompetenciesProfessional ExperiencePublicationsConference PresentationsAcademic and Professional MembershipsResearch and Teaching InterestsCommunity Service, Volunteer Work, and Extra-Curricular ActivitiesAdditional Relevant Skills and LanguagesReferencesIf you dont have relevant information to include beneath any of these section headings, youll want to leave out that section, obviously. However, well go over what each of these sections should include, and show you an example of what it might look like with the correct formatting.P ersonal detailsThis section is the same as how youd begin a resume, with your name, mailing address, telephone number and email address. This information should be in a larger, bolder font compared to the rest of your CV to make it stand out.Joseph A. Smith1555 Main Street, Apartment 212Columbus, Ohio 43004(614) 555-1212 ? joesmith@columbusc.eduEducationIn this section, you should include the academic institutions youve attended, starting with the most recent and working backward. Your high school information should be included, but dont include any information for middle school or elementary school.As with a resume, you should include the school or colleges name, the city and state where it is located, the degree you received (or are currently working on), the Latinate honors you were granted at graduation (if applicable), the program major or concentration, a thesis or dissertation you completed (if applicable), and the dates you attended.Masters in Education, magna cum laude (Jun e 2017)Columbus College, Columbus, OhioThesis title: Reading Strategies for At-Risk, Low-Level ReadersTeaching ExperienceIf youre writing your CV for graduate school, you likely wont have teaching experience to include. However, if this section is relevant and you do have teaching experience to add, include the position, the dates you held it, where the position was held, and a bullet list of all of your duties associated with your job.8th Grade Language Arts Instructor (2016-2017)Columbus City Schools, Columbus, OhioTaught state-mandated objectives in English grammar, writing and literature to students identified as intellectually gifted.Conducted writing workshops for faculty and students focused on state writing assessments; developed professional workshops for faculty members on topics relating to writing and grammar.Maintained classroom grades, records, and parent contact.Research ExperienceHaving strong research skills and experience is highly useful to academic departments lo oking to hire graduate assistants. Its also vital if youre going to be a university professor, as research is required of all tenured academic appointments. Heres an example of what your research experience section might look like:Research Assistant (2015-2016)Department of English, Columbus CollegeAssisted Professor William OReily with primary and secondary source research.Assisted with gathering data and writing methodology of Methods of Making Reading Relevant for At-Risk Boys, published in the Journal of American EducationHonors and AwardsThis is the portion of your CV containing a list of honors and awards youve received. Be sure to include in this section any Presidents List or Deans Lists honors you have earned (along with the years you received them).Award of Excellence in Research, Columbus College, Columbus, OH (2017)Deans List, Columbus College, Columbus, OH (2015-2017)Phi Beta Kappa Award for Outstanding Achievement (2016)Grants AwardedThe standard format for listing any grants you may have received is the following:Current Research (beginning with the most recent one then working backwards)Grant # (PI Name)Name of Funding Organization (avoid using acronyms)Amount AwardedPeriod of Grant AwardTitle of ProjectRole on Project (if not the PI)Technical CompetenciesYour technical competencies are any skills you have beyond your professional and academic experience that would benefit you across industries or roles. For example, your list might look something like this:Project ManagementTechnical WritingData AnalysisData MiningDatabase ManagementStatistical AnalysisProfessional ExperienceYour professional experience is any experience you have earned outside of academic circles, particularly in the working/career world (if both worlds have co-existed for you). As example might be:Assistant Editor (2016-2017)Columbus FlyerConducted interviews and wrote feature articles related to philanthropy, the Arts, events and people in the Greater Columbus area.Copyedit ed each months issue, including calendar/event listings, advertiser contributions and contributions from other writers, ensuring consistency and an error-free publication.Librarian (2010-2013)Columbus Public LibraryShelved books and restocked missing items.Assisted patrons with finding books, researching, paying library fines, using the computers, and looking through microfiche.PublicationsFor this section, you should list your publishing credits. This can include both academic and professional or freelance writing, such as the example below:Smith, J. (2013, June 1). Outreach Opportunities for Volunteers. Columbus Flyer. Retrieved from http://www.columbusflyer.comGrant, P. Smith, J. (2017, March 2). Strategies for Teaching Struggling Readers. Journal of Reading Readiness. Retrieved from http://findjournal.orgConference PresentationsAs with your publishing credits, experiences with conference presentations should be highlighted on your CV, as well. This shows potential employers or academic programs that you are comfortable teaching and instructing others, as well as sharing your findings with relevant audiences. Heres an example of how this section might look:Poster, November 2015: Your Cues In Classroom Management.Undergraduate Educational Arts Symposium, Columbus College.Paper, November 2016: There Are More Ways Than You Can Imagine to Teach a ChildPhi Beta Kappa Annual Conference, Memphis, TN.Academic and Professional MembershipsThis section is a list of the various academic and professional organizations of which you are a member. A longer list shows that you are involved with your field and seek professional development and networking opportunities within it. Your list might look something like this:Modern Language AssociationInternational Sociological AssociationSociety of Young ResearchersPhi Beta Kappa SocietyResearch and Teaching InterestsEspecially if you are creating a CV for graduate school admissions, your audience should be made aware of any res earch or teaching interests you have. Your list might look something like this:At-Risk/Low-Level ReadersContent Area Teaching and LearningCurriculum and InstructionDevelopmental MilestonesDifferentiated InstructionEarly Literacy DevelopmentEducational Technology and Digital MediaEnglish Language LearnersIntervention and PreventionLearning DisabilitiesSustained Silent Reading (SSR)Community Service, Volunteer Work, and Extra-Curricular ActivitiesAdmissions committees and potential employers like to know that you know how to balance your life with activities outside of the classroom or office. This section of your CV might look something like this:Community Volunteer Mentor (2015-2016)Public Libraries of Columbus, OHServed on volunteer committee focused on bringing free mentoring and tutoring services to low-income students.Tutored and mentored low-income, at-risk students needing additional support to succeed in school.Womens Shelter Volunteer (2008-2013)Womens Shelter of ColumbusAss isted with intake of abused and battered women and their children, including counseling, finding suitable clothing and personal toiletry items, scheduling appointments with community outreach services, and leading group sessions.Assisted with shelter upkeep, including cleaning, organization, intake of donations, and food services.Additional Relevant Skills and LanguagesFor this section, list any languages you speak, including whether youre a native speaker or are only fluent in writing it (rather than speaking). The section might look something like this:English (Native)French (Fluent in speaking and writing)ReferencesAs with a resume, including references on your CV allows your audience to contact others to learn more about their experiences working with you or knowing you on a personal level.Tamara Evans, Professor of English,Columbus College(614) 997-8273tamevans@columbusc.eduLacey Smith, Managing EditorColumbus Flyer(614) 543-6372Lacey@columbusflyer.comCV formattingFinally, here are a few additional formatting rules to consider for your CV:Use an 11- or 12- point, easily readable font, such as Times New Roman.Set your margins to approximately one inch.Think like a graphic designer and include enough white space to make sure your CV is easy to read and scan.Be sure to bold, underline, or capitalize important information, but do it consistently.Include a header with your name and page number on each page.Avoid use of abbreviations and acronyms that others might not understand outside of your field.Only print on one side of each sheet of paper.

Essay on Drown - 886 Words

Drown â€Å"The fact that I am writing to you in English already falsifies what I wanted to tell you.†(Diaz) Drown; a compilation of short stories, by Junot Diaz portrays the integration of fiction and truth. Yunior, narrator, as he tells his stories, he exaggerates and jumps from one period of his life to another. The characters of the story can relate to many young adults. Their experiences and the journeys of their lives are what most Hispanic teenagers go through. The 10 different stories explain the different themes shown throughout the book. The Hispanic community faces many problems and Diaz states a couple of them; gender immigration, violence, drugs, family, cultural identity, and the Latin experience. In the beginning,†¦show more content†¦Meanwhile, Rafa sees this as his opportunity to see what is underneath the mask. â€Å"The mask twitched I realized he was smiling and the my brother brought his arm around and smashed the bottle on top of his head.†(Diaz 18) Once the mask was removed, their journey to see what was underneath it was not what they had expected to see. Instead of being happy by their accomplishment they were instead beginning to feel sick. Diaz uses the character of Ysrael to make a comparison with the immigration of lower class individuals. The boys only recognize the familiar and when Ysrael comes along its bizarre for them because they have never seen someone like him. Similarly with lower class people that get ready to face the hardships of getting accustomed to a new language, culture, and environment. It’s tough for immigrants when they first arrive because not only are they faced with learning new traditions but also they have to deal with racism and hatred. Diaz demonstrates the theme of immigration and the hope for the American Dream when the father leaves his family behind and comes to the United States. He promises them that he will send money to them so they can go and be reunited once again. But as soon as he arrives he was forgotten about everything he left in the past. He, like any one, will do anything to be granted citizenship, even if it means cheating on his wife. The father loves his family but he didn’t realize that coming here andShow MoreRelatedDrown895 Words   |  4 PagesDrown The fact that I am writing to you in English already falsifies what I wanted to tell you.(Diaz) Drown; a compilation of short stories, by Junot Diaz portrays the integration of fiction and truth. Yunior, narrator, as he tells his stories, he exaggerates and jumps from one period of his life to another. The characters of the story can relate to many young adults. Their experiences and the journeys of their lives are what most Hispanic teenagers go through. The 10 different storiesRead MoreDrown1334 Words   |  6 Pages In the book Drown by Junot Diaz has expressed a persons experience and environment impacts them in a negative ways. Even the ones that help eachother throughout the way could also affect one in a negative way.It will make one do things one might not want to do and doesnt notice because their in a type of mood. They don t realize what their decisions are taking them the right path taking challenges and overcoming them or go the wrong path to life trying to make things easy for you which meansRead MoreDrown, By Junot Diaz916 Words   |  4 Pages "I usually make a fortune on Saturdays, selling to those kids going down to Belmar or out to Spruce Run.† (Drown). The narrator, Yunior, in the story Drown by Junot Diaz is a drug dealer and does not want anyone to recognize him so that he may uphold a positive image. Unlike Yunior in â€Å"Drown† the narrator in the poem What Work Is by Philip Levine depicts the struggles men and women face when trying to obtain honest work. â€Å"We stand in the rain in a long line waiting at Ford Highland Park, forRead More Drown: A Consideration Essay1484 Words   |  6 Pages In Drown, a collection of short stories, author Junot Diaz presents readers with an impoverished group of characters through harsh, but vivid language. Through the voice of Yunior, the narrator throughout the majority of the stories, Diaz places the blame for Yunior’s negativity and rebellious nature on the disappointment caused by his father and the childhood illusion of America. Diaz, through la nguage and symbolism, forces readers into an emotional bond with Yunior while exposing the illusoryRead MoreAnalysis Of The Poem Drown By Junot Diaz1561 Words   |  7 Pagesto hide their flaws and act a certain way. If men express their un-masculine nature, they will be seen as expressing their weakness. In Drown written by Junot Dà ­az, contains many stories about Dominican Republic male struggles as an immigrant in America. One of the struggles Dà ­az expresses in his text is appearing masculine in society’s eyes. From the story â€Å"Drown†, the unnamed narrator expresses what it means to be masculine. â€Å"Boyfriend† is a story about another unnamed narrator observing his neighbor’sRead MoreHaywood versus Drown Essay2575 Words   |  11 PagesWhy did you choose this civil liability issue? Why did it interest you? In Haywood v. Drown (2008), an inmate (Haywood) is suing a correctional officer (Drown). This civil liability issue is one that is reasonably recent in the field of corrections. Throughout my career thus far, I have been employed as a corrections officer and have heard inmates threatening to sue the department and individual officers. I have also seen countless letters go out to individuals asking for help getting a lawyerRead MoreAnalysis Of Drown By Junot Diaz1073 Words   |  5 Pagesitself. We as a unit give society so much authority that when it comes down to it, we unintentionally allow it to make the decisions for us. This issue becomes very apparent in the movie Moonlight and the collection of short stories â€Å"Drown† by Junot Diaz. In â€Å"Drown†, through the voice the narrator of the stories Yunior, the author Junot Diaz greets the readers with a seemingly very distressed group of characters by vividly describing them with the use of very harsh and viv id language. Junot DiazRead MoreJunot Diaz Drown Research Paper1665 Words   |  7 Pagesimmigrates to a new country they become marginalized, they’re alienated from common cultural practices, social ritual, and scripted behavior. It’s not without intercultural communication and negotiation do immigrants conform to new surroundings. In â€Å"Drown,† the title story of his narrative collection, Junot Diaz enumerates the story of a Hispanic youth growing up in New Jersey. Though Diaz explores issues of queerness, shamelessness, and familial relations within this selection, it is his use of languageRead MoreAnalysis Of Drown By Junot Diazis1461 Words   |  6 PagesIn Drown, by Junot Diaz, there has been many questions about the level of education Yunior has. Although we know he went to college he often shows a very immature side that shows a lack of education. This leads us to think that Yunior is idiotic as he commits a variety of simple-minded acts. But, in fact, Yunior is not as idiotic as we think. What he thinks is usually a lot more intelligent that what he does. This distinction clearly indicates that something else must be guiding Yunior’s decisionsRead MoreAnalysis Of Junot Diaz s Drown 1586 Words   |  7 PagesAlthough most of the short stories Junot Diaz has written are fictionalized the experiences and circumstances described in â€Å"Drown† are authentic. Latinos in neighborhoods all over the world experience the hardships and struggles that Diaz’s characters face in Drown. These characters, like real people are molded and impacted by the issues surrounding them. The dangers found in the neighborhood streets, the exposure to drugs, and the everyday struggles of Latino families prove the impact and authenticity

Pessimistic Nature Of Holden Caulfield In The Catcher In...

Holden Caulfield is an interesting narrator and character of the novel The Catcher in the Rye. Unlike many narrators, he doesn’t seem one-dimensional and possessing only one trait. Many times throughout the novel, Holden’s actions and thoughts tend to conflict with each other, whether that be positive or negative. Therefore, this idea of his more complex nature gives readers differing perspectives of the character and their general preference or distaste of Holden. Regardless of his pessimistic viewpoint of the people around him and his surroundings, I predominately like Holden, specifically for how relatable he is to me and his genuinely caring nature towards certain people. Unfortunately, no person is perfect, and the pessimistic nature†¦show more content†¦As well as this, Holden has a deep desire for the world and the people that inhabit it to slow down. This is evident in the situation Holden finds himself in when looking at the Museum of Natural History, and after describing the fascinating aspects of the museum, he notes that, â€Å"The best thing, though, in that museum was that everything always stayed right where it was† (157). To conclude, Holden has wishes that are common to many people worldwide, therefore making him a relatable character. To add to the positive side of Holden, he does have a capability of caring for other people. Firstly, Holden has a deep love for children and preserving their innocence, to the point that he tells his little sister Phoebe, â€Å"What I have to do, I have to catch everybody if they start to go over the cliff†¦I know it’s crazy, but that’s the only thing I’d really like to be† (224, 225). He wishes to keep children from falling off the cliff of innocence into the real world, and he even does this in a small way by rubbing off a profane message on a wall of the school Phoebe attends. As well as children, Holden has a deep affection for his fellow family members. Throughout theShow MoreRelatedCriticism Of Salinger s The Rye1382 Words   |  6 PagesCRITICISM Salinger’s Catcher in the Rye has been into continuous dispute and controversy since its publication in 1951. Some critics think that Salinger s narrative of the human plight is engrossing and enlightening, yet incredibly depressing. The leading character, Holden Caulfield, serves as the basis for critical discussion due to his psychological conflict. Salinger s portrayal of Holden, which encloses incidents of dejection, nervous breakdown, impulsive spending, sexual exploration, andRead MoreCatcher in the Rye984 Words   |  4 PagesENG 4U1 June 12, 2012 Catcher In The Rye Essay A role model can be can classified as one of many things, but what is it exactly that distinguishes a good one from a bad? The novel, The Catcher in the Rye, written by J.D Salinger is utilized to present the character Holden Caulfield as an unsuitable role model. Firstly, Holden relies on drugs for a way out of his problems instead of facing them. Also, he cannot find his place in the world, which arises, from his natural inclination to lie andRead MoreThe Catcher In The Rye Analysis892 Words   |  4 Pagesany young person. The Catcher in the Rye by J.D. Salinger, illustrates these ideas through Holden Caulfield, his main figure. By displaying how tough it is for him to grow up and admit that he cannot stay adamant in his belief and realize his interpretation of others is limited. The time that is presented allows Holden more ground for stereotypes and explains why his mindset is so set and why he interprets individuals and circumstances in his own sense. The perception of Holden help to present theRead MoreA Pessimistic Adolescent Is Not What Most Consider A Reliable Narrator1295 Words   |  6 PagesA pessimistic adolescent is not what most consider a reliable narrator, but author J.D. Salinger challenges this commonly held thought with his coming-of-age novel The Catcher in the Rye. The reader is introduced to Holden Caulfield, a secondary school junior that is outcasted and aimless upon expulsion from yet another preparatory school in a post-World War II world. Salinger weaves a myriad of comparisons of genuinity and disingenuousness into his work, each explored through a facet of Caulfield’sRead MoreRevealing Caulfield Essay790 Words   |  4 Pages In Salingers The Catcher in the Rye, the physical and psychological unraveling of the narrator, Holden Caulfield, is related through his inarticulate, but sincere voice. Salinger weaves Holdens narration in a way that reflects the young characters age through his words and thoughts. At 16 years yo ung, Holdens narration is often pessimistic, at times enthusiastic, and above all else contradictory. The same could be said about his thoughts concerning the topic of sex, which is a growing sourceRead MoreA Negative View Of Mental Illness1781 Words   |  8 Pagesmakes people think this way? Society has a negative view of mental illness because of media portrayal, the attitudes of individuals, and skepticism, and the novel, The Catcher in the Rye, makes a connection to these views of the world. In the novel The Catcher in the Rye, written by J.D. Salinger, the main character Holden Caulfield is clearly disturbed in some way or another. The opening paragraph begins to paint a clear picture of Holden’s unique and descriptive personality, â€Å"I’ll just tell you

Poem Life and Reality Free Essays

In this poem, the poetess, Elizabeth Sewell, has made an extraordinary resolution to make herself a better woman by facing and living with reality in the start of the New Year. New Year is generally understood as starting of a new chapter of one’s life. People make resolution by taking stock of their lives and resolving to be better ones. We will write a custom essay sample on Poem: Life and Reality or any similar topic only for you Order Now Socrates, the great Greek philosopher, is reported to have said that un-examined life is not worthy of living and it is almost a tradition with the sensible and reasonable people to take stock of their life and to resolve to be better people, at the start of the new year. So, the poetess has decided to live with reality in the New Year. Reality is not always comfortable but pinching and disturbing. The use of the image â€Å"bony arms† points to this fact. But the poetess is resolute and will draw comfort fulfilling her resolution. The phrase ‘draining of long droughts is linked with drinking wine but the poetess intends to drink calmness and thinks it necessary for cleansing herself. It is quite natural and true that in loneliness and quiet one can examine one’s life honestly. It is a self-criticism. Life examined critically purifies one for better future life. I will drain Long draughts of quiet As a purgation; Reference These lines have been taken from ‘New Year resolutions’ written by‘Elizabeth Sewell’. CONTEXT: Elizabeth Sewell determines to be quite so that she may know and judge her self. She may face the reality of life. Explanation In these lines the poetess describes her mental state and the way she would drink to purify her soul. She says that she will drink wine at night secretly. She will have long drops during night and will clean herself from all kinds of evils. This will assist her in helping other people because her own mind and spirit will be free from all types of evils. â€Å"Long draughts of Quiet† also suggests that she will keep quiet most of time. This will be the best means for the purification of her soul and it will help her in leading beneficial and successful life. She says; she will talk less and hear more and mold her life into betterment. Remember Twice daily Who I am; Explanation In the given lines Elizabeth says, she will remember herself twice daily, in the evening and in the morning. She will take into consideration what her aim of creation is. She will try to understand the purpose of life in general. Perhaps she means that she should recognize herself, which will help her in recognizing God and leading life for benefit of others. Will lie o’ nights In the bony arms Of Reality and comforted Explanation These are concluding lines of the poem. Here, the poetess says she will accept reality. While, sleeping at night, she will consider the bitter reality of life. During the new year she will make some promises and pledges. She will sleep on thin, less-fleshy arms of reality. Accepting reality and facts is always a difficult thing, but she will accept it bravely. She will feel relieved and satisfied, when she comes to know that she has spent her life for the benefit of others. The image of bony arms is  a hard reality. Even then she will get delight and feel comforted when she has fulfilled her promises and resolutions, she has made on the start of the new year New In this poem, the poetess, Elizabeth Sewell, has made an extraordinary resolution to make herself a better woman by facing and living with reality in the start of the New Year. New Year is generally understood as starting of a new chapter of one’s life. People make resolution by taking stock of their lives and resolving to be better ones. Socrates, the great Greek philosopher, is reported to have said that unexamined life is not worthy of living and it is almost a tradition with the sensible and reasonable people to take stock of their life and to resolve to be better people, at the start of the new year. So, the poetess has decided to live with reality in the New Year. Reality is not always comfortable but pinching and disturbing. The use of the image â€Å"bony arms† points to this fact. But the poetess is resolute and will draw comfort fulfilling her resolution. The phrase ‘draining of long draughts’ is linked with drinking wine but the poetess intends to drink calmness and thinks it necessary for cleansing herself. It is quite natural and true that in loneliness and quiet one can examine one’s life honestly. It is a self-criticism. Life examined critically purifies one for better future life. Critical Explanation: It is a short and interesting poem written by Elizabeth Sewell. The arrival of New Year is usually celebrated with great fervour and enthusiasm. It is a tradition to make some promises in the light of which one wants to spend one’s New Year. Usually people pledge to become better human beings in the New Year. The poetess makes an extraordinary resolution at the beginning of the New Year. She says that in order to purify her soul, she will remain quiet. She will drink long sips of quietness. It is a beautiful simile as if quietness is a medicine that can make her clean physically and spiritually from impurities. Quietness seems to purify her because when a person is quiet and is not taking active part in the problems of the world, he gets time to have an insight into his own soul. He can then see his own impurities and drawbacks and can get rid of them. The poetess also wants to  do this. She will face the reality. She will not have any high opinion about herself in future. She will not lead a conceited life and have no superiority complex about herself. From now on, she will speak the truth to herself. She will not misjudge her own personality. In order to fulfill this goal. She will remind herself about her own reality twice during a day. Her assessment about herself will be true and real. During the night also, she will not forget the reality. Nodoubt, it is a time when one forgets bitter realities of this world and is lost in the imaginary world of dreams. But she pledges that she will not lose her contact with reality though it is hard like the bony arms yet she will prefer it because she has made her pledge and only the fulfillment of her promise will make her happy. It is very hard thing to face the reality and specially the reality about our own self. Our ego and our conceited self stop us from seeing our weakness. Without facing these realities, we are unable to improve ourselves. So the resolution to see the reality is the first step towards reformation. New 2 â€Å"New Year Resolutions† By Elizabeth Sewell (Reference to the Context) Reference: These lines have been taken from the poem â€Å"New Year Resolutions†, written by Elizabeth Sewell. Context: This poem is about the new year resolutions and promises. Another year has lapsed. On the start of the new year, the poetess takes stock of herself very realistically. She tries to make this world a better place by making new pledges. Her untraditional resolution is to face and lie with reality. Lines 1 – 3 Explanation: In these lines the poetess describes her mental state and the way she would drink to purify her soul. She says that she will drink wine at night secretly. She will have long draughts during night and will purge herself from all kinds of evils. This will assist her in helping other people because her own mind and spirit will be free from all types of evils. â€Å"Long  draughts of Quiet† also suggests that she will keep quiet most of time. This will be the best means for the purification of her oul and it will help her in leading beneficial and successful life. She says; she will talk less and hear more and mould her life into betterment. Lines 4 – 6 In the given lines Elizabeth says, she will remember herself twice daily, in the evening and in the morning. She will take into consideration what her aim of creation is. She will try to understand the purpose of life in general. Perhaps she means that she should recognize herself, which will help her in recognizing God and leading life for benefit of others. Lines 7 – 9 These are concluding lines of the poem. Here, the poetess says she will accept reality. While, sleeping at night, she will consider the bitter reality of life. During the new year she will make some promises and pledges. She will sleep on thin, less-fleshy arms of reality. Accepting reality and facts is always a difficult thing, but she will accept it bravely. She will feel relieved and satisfied, when she comes to know that she has spent her life for the benefit of others. The image of bony arms is a hard reality. Even then she will get delight and feel comforted when she has fulfilled her promises and resolutions, she has made on the start of the new year. My thoughts Explanation In these lines the poetess describes her mental state and the way she would drink to purify her soul. She says that she will drink wine at night secretly. She will have long draughts during night and will purge herself from all kinds of evils.Long draughts of Quiet† also suggests that she will keep quiet most of time. This will be the best means for the purification of her soul and it will help her in leading beneficial and successful life. She says; she will talk less and hear more and mould her life into betterment. Explanation In the given lines Elizabeth says, she will remember herself twice daily, in the evening and in the morning. She will take into consideration what her aim of creation is. She will try to understand the purpose of life in general. Perhaps she means that she should recognize herself, which will help her in recognizing God and leading life for benefit of others. Explanation These are concluding lines of the poem. Here, the poetess says she will accept reality. While, sleeping at night, she will consider the bitter reality of life. During the new year she will make some promises and pledges. She will sleep on thin, less-fleshy arms of reality. Accepting reality and facts is always a difficult thing, but she will accept it bravely. She will feel relieved and satisfied, when she comes to know that she has spent her life for the benefit of others. The image of bony arms is a hard reality. Even then she will get delight and feel comforted when she has fulfilled her promises and resolutions, she has made on the start of the new year These lines have been taken from the poem â€Å"New Year Resolutions†, written byElizabeth Sewell. Context: This poem is about the new year resolutions and promises. Another year has lapsed. On the start of the new year, the poetess takes stock of herself very realistically. She tries to make this world a better place by making new pledges. Her untraditional resolution is to face and lie with reality. Lines 1 – 3 Explanation: In these lines the poetess describes her mental state and the way she would drink to purify her soul. She says that she will drink wine at night secretly. She will have long draughts during night and will purge herself  from all kinds of evils. This will assist her in helping other people because her own mind and spirit will be free from all types ofevils. â€Å"Long draughts of Quiet† also suggests that she will keep quiet most of time. This will be the best means for the purification of her soul and it will help her in leading beneficial and successful life. She says; she will talk less and hear more and mould her life into betterment. Lines 4 – 6 In the given lines Elizabeth says, she will remember herself twice daily, in the eveningand in the morning. She will take into consideration what her aim of creation is. She will try to understand the purpose of life in general. Perhaps she means that she should recognize herself, which will help her in recognizing God and leading life for benefit of others. Lines 7 – 9 These are concluding lines of the poem. Here, the poetess says she will accept reality. While, sleeping at night, she will consider the bitter reality of life. During the new year she will make some promises and pledges. She will sleep on thin, less-fleshy arms of reality. Accepting reality and facts is always a difficult thing, but she will accept it bravely. She will feel relieved and satisfied, when she comes to know that she has spent her life for the benefit of others. The image of bony arms is a hard reality. Even then she will get delight and feel comforted when she has fulfilled her promises and resolutions, she has made on the start of the new year. How to cite Poem: Life and Reality, Essays

Cost Leadership free essay sample

The use of this strategy is primarily to gain an advantage over competitors by reducing operation costs below that of others in the same industry. For example, The Swedish furniture retailer Ikea revolutionized the furniture industry by offering cheap but stylish furniture. Ikea is able to keep its prices low by sourcing its products in low-wage countries and by offering a very basic level of service and it allows Ikea to offer lower prices that attract customer. Differentiation strategy Approach under which a firm aims to develop and market unique products for different customer segments. It is usually employed where a firm has clear competitive advantages, and can sustain an expensive advertising campaign. For example, in consumer electronic there is Sony Company. Sony provide a lot of thing in electronic thing like Vaio for computer, Xperia for hand phone, Sony walkman for music players and etc which they are different from other rival in such their style, specification and quality. We will write a custom essay sample on Cost Leadership or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Focus strategy Refer to the business level strategy based on providing products or services to a specific niche within industry. The firm would narrow its focus on a smaller market niche and decide whether to go for cost leader or differentiation focus. For example firm who decide focus on cost is Mydin that provide low cost product which their focus is for middle class people who can afford their price. While another example for focus on differentiation is Ferrari which have differentiation strategy focus in car industry. They made their product by hand made. Achieving a sustainable competitive advantage over the other competing products and firms in a market is a one of goals for the company. It can be achiece by using Porters Generic Strategies. Diagram above show the three generic business level strategies adopted from Porter, 1985. The three element are cost leadership strategy, differentiation strategy and focus strategy. Firstly, i would like to talk about cost leadership strategy. This strategy used by businesses to create a low cost of operation within their niche. The use of this strategy is primarily to gain an advantage over competitors by reducing operation costs below that of others in the same industry. For example, The Swedish furniture retailer Ikea revolutionized the furniture industry by offering cheap but stylish furniture. Ikea is able to keep its prices low by sourcing its products in low-wage countries and by offering a very basic level of service and it allows Ikea to offer lower prices that attract customer. Secondly, differentiation strategy approach under which a firm aims   to develop and market unique product for different customer segment. It is usually employed here a firm has clear competitive advantages and can sustain an expensive advertising campaign. For example, in consumer electronic there is Sony Company. Sony provide a lot of thing in electronic thing like Vaio for computer, Xperia for hand phone, Sony walkman for music players and etc which they are different from other rival in such their style, specification and quality. Last strategy is focus strategy which refer to the business level strategy based on providing products or services to a specific niche within industry. It also diveded into two form which cost based focus and differentiation focus. The firm would narrow its focus on a smaller market niche and decide whether to go for cost leader or differentiation focus. For example firm who decide focus on cost is Mydin, a grocery stores   that provide low cost product which their focus is for middle class people who can afford their price. While another example for focus on differentiation is Ferrari which have differentiation strategy focus in car industry. They made their product by hand made and their focus are for rich people who are willing to pay a high price in order to get differentiation from others. As conclusion, here are some strategies that firm can take in order to achieve sustainable competitive advantage  over the other competing products and firms in a market which using Porters Generic Strategies and some of example from me to make you all have firm to make sample for reference. I hope you can understand and can use it as a new plan strategies in order to achieve sustainable competitive advantage over the other competing products and firms in a market.